SpectraComp®

SpectraComp® eGFP Controls are precision engineered multi level fluorescent cell mimics designed to support accurate unmixing, compensation and calibration of GFP signals in multiparameter flow cytometry workflows. These controls present eGFP protein on the particle surface and provide negative, low, medium and high fluorescence populations that mimic the performance of eGFP expressing cells. Each positive population contains a defined number of eGFP molecules, generating distinct fluorescence intensity levels for reliable benchmarking and reference signal generation. With consistent fluorescence intensity, cell-like autofluorescence and the high performance characteristics shared across all SpectraComp Controls, SpectraComp eGFP delivers dependable signal resolution and reduces errors associated with spectral overlap. The kit includes four liquid dropper vials containing separate negative, low, medium and high populations, supporting flexible experimental setup and streamlined workflow integration. These controls are suitable for spectral unmixing, conventional compensation, fluorescence calibration and assessment of transfection efficiency. SpectraComp eGFP requires no special handling or safety precautions and has been validated on analytical flow cytometers for dependable performance in daily QC, assay development and high parameter panel design. Unit Size: 25 tests of each populationPackaging: separate neg, low, med, and high populationsProduct format: 4 liquid dropper vialsApplication: Unmixing, compensation, calibration, or transfection efficiency control Benefits: State-of-the-art controls designed using actual eGFP protein (excitation peak at 488 nm and max emission at 507 nm) Three mean fluorescence intensity levels (low, medium, and high) Consistent fluorescence signals for reliable performance Cell-like autofluorescence for workflow efficiency Resources: Technical Data Sheet Safety Data Sheet Certificate of Analysis

SpectraComp® Flex Controls are precision engineered single stain cell mimics designed to optimize compensation and support accurate spectral unmixing in multiparameter flow cytometry workflows. These controls mimic the staining performance of real cells and provide a clear positive fluorescence histogram after staining with fluorescently conjugated antibodies. Each control captures antibodies from mouse, rat and hamster sources, supporting broad panel compatibility and diverse fluorophore selections. With unparalleled high fluorescence response and cell-like autofluorescence, SpectraComp Flex Controls deliver superior signal to noise resolution and reduces errors associated with spectral overlap. The consistent fluorescence intensity and physiologically relevant scatter characteristics of all SpectraComp Controls outperform traditional polystyrene based beads and support dependable compensation across instruments and platforms. To further support flexible experiment setup, SpectraComp Flex is supplied in separate positive and negative vials. These controls require no special handling or safety precautions and have been validated on analytical flow cytometers for reliable performance in daily QC, assay development and high parameter panel design. Benefits: Unparalleled high fluorescence response (greater than 10e5 MFI on most bright fluorochromes) Conveniently works with multiple species of antibodies: mouse, rat, and hamster Exceptional performance for compensation across instrument platforms Compatible with violet, UV fluorochrome, and tandem dyes Cell-like autofluorescence for superior signal-to-noise resolution Resources: Technical Data Sheet Safety Data Sheet Certificate of Analysis Poster: Spectral Cytometry Reference Controls: The Search for the Perfect Particle Publication: Jensen, HA, Kim, J. iCoreDrop: A robust immune monitoring spectral cytometry assay with six open channels for biomarker flexibility. Cytometry. 2023; 103(5): 405–418. https://doi.org/10.1002/cyto.a.24708

SpectraComp® Flex Multipeak Controls are precision engineered hydrogel based single stain controls designed to support accurate compensation, spectral unmixing and fluorescence standardization in multiparameter flow cytometry workflows. These hydrogels capture antibodies from mouse, rat, hamster and rabbit sources and provide three defined levels of antibody binding — negative, medium and high — enabling multi level reference signals that mimic the fluorescence spectra of stained cells across multiple antigen density levels. With the consistent fluorescence intensity, cell-like autofluorescence and broad antibody species compatibility characteristic of all SpectraComp Controls, SpectraComp Flex Multipeak outperforms traditional bead based tools and reduces errors associated with spectral overlap. Each kit includes a negative bottle containing a 100% negative population and a positive bottle containing a 50% high and 50% medium population. After staining with fluorescently conjugated antibodies, the hydrogels yield a fluorescence histogram with two positive peaks and one negative peak, supporting clear fluorophore performance assessment and reliable single stain reference signals for compensation and unmixing. These controls require no special handling or safety precautions and have been validated on analytical flow cytometers for dependable performance in daily QC and long term panel design. The product contains: Hydrogels with 3 levels of antibody capture: Negative Medium High Each kit comes with 2 dropper bottles (liquid) Negative Bottle: 100% negative population 100,000 hydrogels per drop Positive Bottle: 50% High / 50% Medium 100,000 hydrogels per drop (50,000 of each population) SpectraComp® Flex Multipeak can be used for compensation or spectral unmixing or as an instrument standardization control for fluorescence. Resources Technical Data Sheet Safety Data Sheet Certificate of Analysis