SpectraComp® controls are precision engineered single stain cell mimics designed to optimize spectral unmixing and compensation in multiparameter flow cytometry workflows. These controls mimic the staining performance of real cells and provide positive and negative populations after staining with fluorescently conjugated antibodies. Each control captures antibodies from mouse, rat and hamster sources, supporting broad panel compatibility and diverse fluorophore selections.
With high fluorescence response and cell like autofluorescence, SpectraComp delivers superior signal to noise resolution and reduces errors associated with spectral overlap. Its consistent fluorescence intensity and physiologically relevant scatter characteristics outperform traditional polystyrene based beads while preserving the behavior of cell based controls. SpectraComp is suitable for spectral unmixing or conventional compensation and is supplied as a single vial containing both positive and negative capture mimics for streamlined workflows.
These controls require no special handling or safety precautions and have been validated on analytical flow cytometers for dependable performance in assay development and high parameter panel design.
| Specification | Value |
|---|---|
| Format | Single vial containing positive and negative capture mimics |
| Available Sizes | 25 tests (SSB-05-A), 100 tests (SSB-05-B) |
| Species Compatibility | Mouse, rat, hamster |
| Fluorochrome Compatibility | Compatible with violet and UV fluorochromes and tandem dyes |
| Application | Spectral unmixing and conventional compensation in multiparameter flow cytometry |
| Instrument Compatibility | Validated on analytical flow cytometers; compatible across multiple instrument platforms |
| Handling & Safety | No special handling or safety precautions required |
| Manufacturer | Slingshot Biosciences |
Spectral Cytometry Reference Controls Poster
Spectral Cytometry Reference Controls: The Search for the Perfect Particle
iCorp Case Study
iCoreDrop: A robust immune monitoring spectral cytometry assay
Workflow for Spectral Compensation
A Workflow for Spectral Compensation with SpectraComp Synthetic Cell Controls
SpectraComp® Controls are precision engineered single stain cell mimics designed to optimize spectral unmixing and compensation in multiparameter flow cytometry workflows. These controls mimic the staining performance of real cells and provide positive and negative fluorescence peaks after staining with fluorescently conjugated antibodies. Each control captures antibodies from mouse, rat and hamster sources, supporting broad panel compatibility and diverse fluorophore selections. With high fluorescence response and cell like autofluorescence, SpectraComp delivers superior signal to noise resolution and reduces errors associated with spectral overlap. Its consistent fluorescence intensity and physiologically relevant scatter characteristics outperform traditional polystyrene based beads while preserving the behavior of cell based controls. SpectraComp is suitable for spectral unmixing or conventional compensation and is supplied as a single vial containing both positive and negative capture mimics for streamlined workflows. These controls require no special handling or safety precautions and have been validated on analytical flow cytometers for dependable performance in daily QC, assay development and high parameter panel design. Benefits: Unparalleled high fluorescence response (greater than 10e5 MFI on most bright fluorochrome) Conveniently works with multiple species of antibodies: mouse, rat, and hamster Exceptional performance in spectral analysis and unmixing Compatible with violet and UV fluorochromes Cell-like autofluorescence for superior signal-to-noise resolution Resources: SpectraComp® Application Note Technical Data Sheet Safety Data Sheet Certificate of Analysis Poster: Spectral Cytometry Reference Controls: The Search for the Perfect Particle Publication: Jensen, HA, Kim, J. iCoreDrop: A robust immune monitoring spectral cytometry assay with six open channels for biomarker flexibility. Cytometry. 2023; 103(5): 405–418. https://doi.org/10.1002/cyto.a.24708
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