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BLOG: TBNK Immunophenotyping Controls: Biological vs. Synthetic Reference Materials
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Nature varies. Plastic pretends. We don't. Slingshot Bio. Certainty.

Plastic isn’t biology, donors aren’t reproducible, and animals aren’t human—so stop compromising. Bring high-precision cell mimics into your flow cytometry workflow to achieve consistent, reliable results at every step.

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CD34+ Stem Cell Mimic™

CD34+ Stem Cell Mimic™ is a lyophilized cell mimic designed for reliable performance checks and normalization controls in immunophenotyping and flow cytometry workflows. This product features CD34, CD45 and DNA‑based viability biomarkers with scatter characteristics that closely resemble human lymphocyte populations. It provides a high density CD34+ population with frequencies comparable to biological samples such as cord blood, bone marrow and mobilized peripheral blood cells (~90% CD34+, as a percentage of CD45+ cells), enabling confident population discrimination and gating.

The control is intended to provide positive signal detection for CD45 and both positive and negative signal detection for CD34 and viability. It supports robust gating strategies for CD34 enumeration and viability assessment and serves as a dependable tool for antibody qualification and staining verification. The kit comprises three lymphocyte‑mimic populations designed for clear transferable gates: CD45+ cells, CD45+/CD34+ cells and CD45+ cells with a viability readout. This well‑defined population structure supports accurate gating, viability discrimination and instrument verification across daily QC, assay validation and longitudinal performance monitoring. The lyophilized format ensures consistent reconstitution and extended shelf life, making the CD34+ Stem Cell Mimics suitable for multi‑site standardization and long‑term assay harmonization across diverse cytometry platforms.

SpecificationValue
FormatLyophilized cell mimic; three lymphocyte-mimic populations (CD45+, CD45+/CD34+, and CD45+ with viability readout)
Available Sizes1 kit (SL-00046)
BiomarkersCD34, CD45, DNA-based viability
CD34+ Frequency~90% CD34+ as a percentage of CD45+ cells — comparable to cord blood, bone marrow, and mobilized peripheral blood
Scatter CharacteristicsClosely resembles human lymphocyte populations
ApplicationCD34 enumeration, viability assessment, antibody qualification, staining verification, immunophenotyping, and flow cytometry QC
Instrument CompatibilityCompatible across diverse cytometry platforms; supports multi-site standardization
Handling & SafetyNo special handling or safety precautions required
Shipping TempWet Ice
Shelf Life12 months
ManufacturerSlingshot Biosciences

CD34+ Stem Cell Mimic™

Quantity
1
Price$324.00

Product documentation

Technical Data SheetCertificate of Analysis Safety Data Sheets

No frequently asked questions for this product yet.

Posters4
Poster

CD34 Stem Cell Mimics as reliable controls for Cell Therapy: Addressing challenges in CD34 expression in Stem Cells and Lot-to-Lot Consistency

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Poster

Cell Mimics as Robust Positive Controls for Immunodeficiency Analyses

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Poster

CD34 Stem Cell Mimics as reliable controls for Cell Therapy

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Poster

Hydrogels Mimicking T, B and NK Cells for Quality Assurance in Cell Therapy Development

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Application Notes2
Application Note

Use of CD34+ Stem Cell Mimic for assay sensitivity testing

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Application Note

TBNK Mimic as a Reliable Alternative to PBMC Controls for Consistent Immunophenotyping in Flow Cytometry

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Published Articles1
Published Article

Evaluation of Cell Mimics as Potential Quality Controls for Human Leukocyte Immunophenotyping

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