SpectraComp® Flex controls are precision engineered single stain cell mimics designed to optimize compensation and support accurate spectral unmixing in multiparameter flow cytometry workflows. These controls mimic the staining performance of real cells and provide a clear positive population after staining with fluorescently conjugated antibodies. Each control captures antibodies from mouse, rat and hamster sources, supporting broad panel compatibility and diverse fluorophore selections.
With unparalleled high fluorescence response and cell-like autofluorescence, SpectraComp Flex controls deliver superior signal to noise resolution and reduces errors associated with spectral overlap. The consistent fluorescence intensity and physiologically relevant scatter characteristics of all SpectraComp controls outperform traditional polystyrene based beads and support dependable compensation and spectral unmixing across instruments and platforms. To support flexible experiment setup, SpectraComp Flex is supplied in separate positive and negative vials.
These controls require no special handling or safety precautions and have been validated on analytical flow cytometers for reliable performance in, assay development and high parameter panel design.
| Specification | Value |
|---|---|
| Format | Separate positive and negative vials |
| Available Sizes | 100 tests (SSB-20-A) |
| Species Compatibility | Mouse, rat, hamster |
| Fluorochrome Compatibility | Compatible with violet and UV fluorochromes and tandem dyes |
| Application | Spectral unmixing and conventional compensation in multiparameter flow cytometry |
| Instrument Compatibility | Validated on analytical flow cytometers; compatible across multiple instrument platforms |
| Handling & Safety | No special handling or safety precautions required |
| Manufacturer | Slingshot Biosciences |
SpectraComp® Flex Controls are precision engineered single stain cell mimics designed to optimize compensation and support accurate spectral unmixing in multiparameter flow cytometry workflows. These controls mimic the staining performance of real cells and provide a clear positive fluorescence histogram after staining with fluorescently conjugated antibodies. Each control captures antibodies from mouse, rat and hamster sources, supporting broad panel compatibility and diverse fluorophore selections. With unparalleled high fluorescence response and cell-like autofluorescence, SpectraComp Flex Controls deliver superior signal to noise resolution and reduces errors associated with spectral overlap. The consistent fluorescence intensity and physiologically relevant scatter characteristics of all SpectraComp Controls outperform traditional polystyrene based beads and support dependable compensation across instruments and platforms. To further support flexible experiment setup, SpectraComp Flex is supplied in separate positive and negative vials. These controls require no special handling or safety precautions and have been validated on analytical flow cytometers for reliable performance in daily QC, assay development and high parameter panel design. Benefits: Unparalleled high fluorescence response (greater than 10e5 MFI on most bright fluorochromes) Conveniently works with multiple species of antibodies: mouse, rat, and hamster Exceptional performance for compensation across instrument platforms Compatible with violet, UV fluorochrome, and tandem dyes Cell-like autofluorescence for superior signal-to-noise resolution Resources: Technical Data Sheet Safety Data Sheet Certificate of Analysis Poster: Spectral Cytometry Reference Controls: The Search for the Perfect Particle Publication: Jensen, HA, Kim, J. iCoreDrop: A robust immune monitoring spectral cytometry assay with six open channels for biomarker flexibility. Cytometry. 2023; 103(5): 405–418. https://doi.org/10.1002/cyto.a.24708
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