QuantCyte™ CD19 quantitation controls are precision engineered lyophilized cell mimics designed to quantify CD19 molecules per cell and support consistent expression benchmarking across research and development workflows. These synthetic cells feature the CD19 biomarker with scatter properties resembling human lymphocytes, enabling realistic assay performance and reliable calibration.
Each kit provides four defined populations representing negative, low, medium and high CD19 expression levels. Each positive population contains a known number of CD19 molecules, generating distinct fluorescence intensities and antigen density levels for accurate quantification. Using a PE or APC conjugated CD19 antibody, these controls allow direct measurement of antibody binding capacity without requiring knowledge of fluorophore molecules per antibody. Incorporating the measured intensities into the provided Antigen Density Calculator enables generation of a signal to antigen standard curve that can be used to quantify antigen densities on cell targets with the same instrumentation and antibody.
As with all QuantCyte products, QuantCyte CD19 quantitation controls are safe to use in any environment, contain no biohazardous material and require no special disposal.
| Specification | Value |
|---|---|
| Format | Lyophilized synthetic cell mimics; four defined populations (negative, low, medium, and high CD19 expression) |
| Available Sizes | 1 kit (NPI-00036) |
| Target Biomarker | CD19 |
| Recommended Antibody Conjugates | PE or APC conjugated CD19 antibody |
| Application | CD19 antigen density quantification, antibody binding capacity measurement, expression benchmarking, and signal-to-antigen standard curve generation |
| Scatter Characteristics | Resembles human lymphocyte populations |
| Instrument Compatibility | Compatible across multiple analytical platforms |
| Handling & Safety | No biohazardous material; no special handling, disposal, or safety precautions required |
| Included Tools | Antigen Density Calculator (Excel) for standard curve generation |
| Manufacturer | Slingshot Biosciences |
QuantCytes™ CD19 quantitation controls are precision engineered lyophilized cell mimics designed to quantify CD19 molecules per cell and support consistent expression benchmarking across research and development workflows. These synthetic cells feature the CD19 biomarker with scatter properties resembling human lymphocytes, enabling realistic assay performance and reliable calibration. Each kit provides four defined populations representing negative, low, medium and high CD19 expression levels. Each positive population contains a known number of CD19 molecules, generating distinct fluorescence intensities and antigen density levels for accurate quantification. Using a PE or APC conjugated CD19 antibody, these controls allow direct measurement of antibody binding capacity without requiring knowledge of fluorophore molecules per antibody. Incorporating the measured intensities into the provided Antigen Density Calculator enables generation of a signal to antigen standard curve that can be used to quantify antigen densities on cell targets with the same instrumentation and antibody. As with all QuantCytes™ products, QuantCytes™ CD19 quantitation controls are safe to use in any environment, contain no biohazardous material and require no special disposal. ResourcesTechnical Data Sheet Certificate of Analysis Antigen Density Calculator Poster: Enhancing Immunophenotyping Standardization in Clinical Labs with Quantitative BCMA or CD19 Cell Mimics
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