SpectraComp® eGFP controls are precision engineered multi level fluorescent cell mimics designed to support accurate unmixing, compensation and calibration of GFP signals in multiparameter flow cytometry workflows. These controls present eGFP protein on the particle surface and provide negative, low, medium and high fluorescence populations that mimic the performance of eGFP expressing cells. Each positive population contains a defined number of eGFP molecules, generating distinct fluorescence intensity levels for reliable benchmarking and reference signal generation.
With consistent fluorescence intensity, cell-like autofluorescence and the high performance characteristics shared across all SpectraComp controls, SpectraComp eGFP delivers dependable signal resolution and reduces errors associated with spectral overlap. The kit includes four liquid dropper vials containing separate negative, low, medium and high populations, supporting flexible experimental setup and streamlined workflow integration. These controls are suitable for spectral unmixing, conventional compensation, fluorescence calibration and assessment of transfection efficiency.
SpectraComp eGFP controls require no special handling or safety precautions and has been validated on analytical flow cytometers for dependable performance in assay development and high parameter panel design.
| Specification | Value |
|---|---|
| Format | 4 liquid dropper vials; separate negative, low, medium and high eGFP populations |
| Available Sizes | 25 tests per population (SSB-23-A) |
| Fluorophore | eGFP protein (excitation peak 488 nm; max emission 507 nm) |
| Fluorescence Levels | 4 defined levels: negative, low, medium and high — each containing a known number of eGFP molecules |
| Scatter Characteristics | Cell-like autofluorescence and physiologically relevant scatter properties |
| Application | Spectral unmixing, conventional compensation, fluorescence calibration, transfection efficiency assessment, and daily QC |
| Instrument Compatibility | Validated on analytical flow cytometers; compatible across multiple instrument platforms |
| Handling & Safety | No special handling or safety precautions required |
| Manufacturer | Slingshot Biosciences |
SpectraComp® eGFP Controls are precision engineered multi level fluorescent cell mimics designed to support accurate unmixing, compensation and calibration of GFP signals in multiparameter flow cytometry workflows. These controls present eGFP protein on the particle surface and provide negative, low, medium and high fluorescence populations that mimic the performance of eGFP expressing cells. Each positive population contains a defined number of eGFP molecules, generating distinct fluorescence intensity levels for reliable benchmarking and reference signal generation. With consistent fluorescence intensity, cell-like autofluorescence and the high performance characteristics shared across all SpectraComp Controls, SpectraComp eGFP delivers dependable signal resolution and reduces errors associated with spectral overlap. The kit includes four liquid dropper vials containing separate negative, low, medium and high populations, supporting flexible experimental setup and streamlined workflow integration. These controls are suitable for spectral unmixing, conventional compensation, fluorescence calibration and assessment of transfection efficiency. SpectraComp eGFP requires no special handling or safety precautions and has been validated on analytical flow cytometers for dependable performance in daily QC, assay development and high parameter panel design. Unit Size: 25 tests of each populationPackaging: separate neg, low, med, and high populationsProduct format: 4 liquid dropper vialsApplication: Unmixing, compensation, calibration, or transfection efficiency control Benefits: State-of-the-art controls designed using actual eGFP protein (excitation peak at 488 nm and max emission at 507 nm) Three mean fluorescence intensity levels (low, medium, and high) Consistent fluorescence signals for reliable performance Cell-like autofluorescence for workflow efficiency Resources: Technical Data Sheet Safety Data Sheet Certificate of Analysis
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