SpectraComp® Flex Multipeak controls are precision engineered hydrogel based single stain controls designed to support accurate compensation, spectral unmixing and fluorescence standardization in multiparameter flow cytometry workflows. These hydrogels capture antibodies from mouse, rat, hamster and rabbit sources and provide three defined levels of antibody binding — negative, medium and high — enabling multi level reference signals that mimic the fluorescence spectra of stained cells across multiple antigen density levels.
With the consistent fluorescence intensity, cell-like autofluorescence and broad antibody species compatibility characteristic of all SpectraComp controls, SpectraComp Flex Multipeak outperforms traditional bead based tools and reduces errors associated with spectral overlap. Each kit includes a negative bottle containing a 100% negative population and a positive bottle containing a 50% high and 50% medium population. After staining with fluorescently conjugated antibodies, the hydrogels yield a fluorescence output with two positive populations and one negative population, supporting clear fluorophore performance assessment and reliable single stain reference signals for compensation and spectral unmixing.
These controls require no special handling or safety precautions and have been validated on analytical flow cytometers for dependable performance in assay development and high parameter panel design.
| Specification | Value |
|---|---|
| Format | Hydrogel-based; 2 liquid dropper bottles — negative bottle (100% negative, 100,000 hydrogels/drop) and positive bottle (50% high / 50% medium, 100,000 hydrogels/drop) |
| Available Sizes | 50 tests (SL-00028-A) |
| Capture Levels | 3 defined levels: negative, medium, and high antibody binding |
| Species Compatibility | Mouse, rat, hamster, rabbit |
| Fluorochrome Compatibility | Compatible with violet and UV fluorochromes and tandem dyes |
| Application | Compensation, spectral unmixing, fluorescence standardization, instrument QC, and long-term panel design |
| Histogram Output | Two positive peaks (high and medium) and one negative peak per single stain |
| Instrument Compatibility | Validated on analytical flow cytometers; compatible across multiple instrument platforms |
| Handling & Safety | No special handling or safety precautions required |
| Manufacturer | Slingshot Biosciences |
SpectraComp® Flex Multipeak Controls are precision engineered hydrogel based single stain controls designed to support accurate compensation, spectral unmixing and fluorescence standardization in multiparameter flow cytometry workflows. These hydrogels capture antibodies from mouse, rat, hamster and rabbit sources and provide three defined levels of antibody binding — negative, medium and high — enabling multi level reference signals that mimic the fluorescence spectra of stained cells across multiple antigen density levels. With the consistent fluorescence intensity, cell-like autofluorescence and broad antibody species compatibility characteristic of all SpectraComp Controls, SpectraComp Flex Multipeak outperforms traditional bead based tools and reduces errors associated with spectral overlap. Each kit includes a negative bottle containing a 100% negative population and a positive bottle containing a 50% high and 50% medium population. After staining with fluorescently conjugated antibodies, the hydrogels yield a fluorescence histogram with two positive peaks and one negative peak, supporting clear fluorophore performance assessment and reliable single stain reference signals for compensation and unmixing. These controls require no special handling or safety precautions and have been validated on analytical flow cytometers for dependable performance in daily QC and long term panel design. The product contains: Hydrogels with 3 levels of antibody capture: Negative Medium High Each kit comes with 2 dropper bottles (liquid) Negative Bottle: 100% negative population 100,000 hydrogels per drop Positive Bottle: 50% High / 50% Medium 100,000 hydrogels per drop (50,000 of each population) SpectraComp® Flex Multipeak can be used for compensation or spectral unmixing or as an instrument standardization control for fluorescence. Resources Technical Data Sheet Safety Data Sheet Certificate of Analysis
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