SpectraComp® Flex Multipeak controls are precision engineered hydrogel based single stain controls designed to support accurate compensation, spectral unmixing and fluorescence standardization in multiparameter flow cytometry workflows. These hydrogels capture antibodies from mouse, rat, hamster and rabbit sources and provide three defined levels of antibody binding — negative, medium and high — enabling multi level reference signals that mimic the fluorescence spectra of stained cells across multiple antigen density levels.
With the consistent fluorescence intensity, cell-like autofluorescence and broad antibody species compatibility characteristic of all SpectraComp controls, SpectraComp Flex Multipeak outperforms traditional bead based tools and reduces errors associated with spectral overlap. Each kit includes a negative bottle containing a 100% negative population and a positive bottle containing a 50% high and 50% medium population. After staining with fluorescently conjugated antibodies, the hydrogels yield a fluorescence output with two positive populations and one negative population, supporting clear fluorophore performance assessment and reliable single stain reference signals for compensation and spectral unmixing.
These controls require no special handling or safety precautions and have been validated on analytical flow cytometers for dependable performance in assay development and high parameter panel design.
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SpectraComp® Flex Multipeak
SpectraComp® Flex Multipeak
Specifications
| Specification | Value |
|---|---|
| Format | Hydrogel-based; 2 liquid dropper bottles — negative bottle (100% negative, 100,000 hydrogels/drop) and positive bottle (50% high / 50% medium, 100,000 hydrogels/drop) |
| Available Sizes | 50 tests (SL-00028-A) |
| Capture Levels | 3 defined levels: negative, medium, and high antibody binding |
| Species Compatibility | Mouse, rat, hamster, rabbit |
| Fluorochrome Compatibility | Compatible with violet and UV fluorochromes and tandem dyes |
| Application | Compensation, spectral unmixing, fluorescence standardization, instrument QC, and long-term panel design |
| Histogram Output |
Datasheets & certificates
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Questions
Yes. The product is built to behave the same on conventional and spectral platforms — BD, Cytek, Beckman, the major systems. The single-stain reference unmixes cleanly across BV, BUV, and Super Bright dyes, which is usually where mismatched controls fall apart. If your core has something unusual on the floor, ask us — we've probably already run on it.
From the community
| Two positive peaks (high and medium) and one negative peak per single stain |
| Instrument Compatibility | Validated on analytical flow cytometers; compatible across multiple instrument platforms |
| Handling & Safety | No special handling or safety precautions required |
| Storage Temp | 2 - 8°C |
| Shipping Temp | No Ice |
| Shelf Life | 18 months |
| Guaranteed Expiration Date | >6 months at shipping |
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One single-stain control for amine-reactive viability dyes and fluorophore-conjugated antibodies. Cell-like scatter, non-biohazardous, ready-to-use dropper format.
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Slingshot Bio Reagent
SpectraComp® Controls are precision engineered single stain cell mimics designed to optimize spectral unmixing and compensation in multiparameter flow cytometry workflows. These controls mimic the staining performance of real cells and provide positive and negative fluorescence peaks after staining with fluorescently conjugated antibodies. Each control captures antibodies from mouse, rat and hamster sources, supporting broad panel compatibility and diverse fluorophore selections. With high fluorescence response and cell like autofluorescence, SpectraComp delivers superior signal to noise resolution and reduces errors associated with spectral overlap. Its consistent fluorescence intensity and physiologically relevant scatter characteristics outperform traditional polystyrene based beads while preserving the behavior of cell based controls. SpectraComp is suitable for spectral unmixing or conventional compensation and is supplied as a single vial containing both positive and negative capture mimics for streamlined workflows. These controls require no special handling or safety precautions and have been validated on analytical flow cytometers for dependable performance in daily QC, assay development and high parameter panel design. Benefits: Unparalleled high fluorescence response (greater than 10e5 MFI on most bright fluorochrome) Conveniently works with multiple species of antibodies: mouse, rat, and hamster Exceptional performance in spectral analysis and unmixing Compatible with violet and UV fluorochromes Cell-like autofluorescence for superior signal-to-noise resolution Resources: SpectraComp® Application Note Technical Data Sheet Safety Data Sheet Certificate of Analysis Poster: Spectral Cytometry Reference Controls: The Search for the Perfect Particle Publication: Jensen, HA, Kim, J. iCoreDrop: A robust immune monitoring spectral cytometry assay with six open channels for biomarker flexibility. Cytometry. 2023; 103(5): 405–418. https://doi.org/10.1002/cyto.a.24708
Slingshot Bio Reagent
SpectraComp® eGFP Controls are precision engineered multi level fluorescent cell mimics designed to support accurate unmixing, compensation and calibration of GFP signals in multiparameter flow cytometry workflows. These controls present eGFP protein on the particle surface and provide negative, low, medium and high fluorescence populations that mimic the performance of eGFP expressing cells. Each positive population contains a defined number of eGFP molecules, generating distinct fluorescence intensity levels for reliable benchmarking and reference signal generation. With consistent fluorescence intensity, cell-like autofluorescence and the high performance characteristics shared across all SpectraComp Controls, SpectraComp eGFP delivers dependable signal resolution and reduces errors associated with spectral overlap. The kit includes four liquid dropper vials containing separate negative, low, medium and high populations, supporting flexible experimental setup and streamlined workflow integration. These controls are suitable for spectral unmixing, conventional compensation, fluorescence calibration and assessment of transfection efficiency. SpectraComp eGFP requires no special handling or safety precautions and has been validated on analytical flow cytometers for dependable performance in daily QC, assay development and high parameter panel design. Unit Size: 25 tests of each populationPackaging: separate neg, low, med, and high populationsProduct format: 4 liquid dropper vialsApplication: Unmixing, compensation, calibration, or transfection efficiency control Benefits: State-of-the-art controls designed using actual eGFP protein (excitation peak at 488 nm and max emission at 507 nm) Three mean fluorescence intensity levels (low, medium, and high) Consistent fluorescence signals for reliable performance Cell-like autofluorescence for workflow efficiency Resources: Technical Data Sheet Safety Data Sheet Certificate of Analysis
Slingshot Bio Reagent
SpectraComp® Flex Controls are precision engineered single stain cell mimics designed to optimize compensation and support accurate spectral unmixing in multiparameter flow cytometry workflows. These controls mimic the staining performance of real cells and provide a clear positive fluorescence histogram after staining with fluorescently conjugated antibodies. Each control captures antibodies from mouse, rat and hamster sources, supporting broad panel compatibility and diverse fluorophore selections. With unparalleled high fluorescence response and cell-like autofluorescence, SpectraComp Flex Controls deliver superior signal to noise resolution and reduces errors associated with spectral overlap. The consistent fluorescence intensity and physiologically relevant scatter characteristics of all SpectraComp Controls outperform traditional polystyrene based beads and support dependable compensation across instruments and platforms. To further support flexible experiment setup, SpectraComp Flex is supplied in separate positive and negative vials. These controls require no special handling or safety precautions and have been validated on analytical flow cytometers for reliable performance in daily QC, assay development and high parameter panel design. Benefits: Unparalleled high fluorescence response (greater than 10e5 MFI on most bright fluorochromes) Conveniently works with multiple species of antibodies: mouse, rat, and hamster Exceptional performance for compensation across instrument platforms Compatible with violet, UV fluorochrome, and tandem dyes Cell-like autofluorescence for superior signal-to-noise resolution Resources: Technical Data Sheet Safety Data Sheet Certificate of Analysis Poster: Spectral Cytometry Reference Controls: The Search for the Perfect Particle Publication: Jensen, HA, Kim, J. iCoreDrop: A robust immune monitoring spectral cytometry assay with six open channels for biomarker flexibility. Cytometry. 2023; 103(5): 405–418. https://doi.org/10.1002/cyto.a.24708
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