SpectraComp® XT controls are precision engineered single stain cell mimics designed to optimize spectral unmixing and compensation in multiparameter flow cytometry workflows. These controls mimic the staining performance of real cells and provide clear positive and negative fluorescence peaks after staining with fluorescently conjugated antibodies.
As with all SpectraComp controls, SpectraComp XT delivers high fluorescence response and cell-like autofluorescence to provide superior signal to noise resolution and reduce errors associated with spectral overlap. The consistent fluorescence intensity and physiologically relevant scatter characteristics of SpectraComp controls outperform traditional polystyrene based beads and support reliable spectral unmixing or conventional compensation across instruments and platforms. SpectraComp XT expands species compatibility by capturing antibodies from mouse, rat, hamster, rabbit and human sources, supporting broad panel design and diverse fluorophore selections. Compatible with violet, UV and tandem dyes, these controls are supplied as a combined positive and negative population for streamlined workflow integration.
These controls require no special handling or safety precautions and have been validated on analytical flow cytometers for dependable performance in assay development and high parameter panel design.
| Specification | Value |
|---|---|
| Format | Single vial containing combined positive and negative populations |
| Available Sizes | 50 tests (SSB-21-A) |
| Species Compatibility | Mouse, rat, hamster, rabbit, human |
| Fluorochrome Compatibility | Compatible with violet and UV fluorochromes and tandem dyes |
| Application | Spectral unmixing and conventional compensation in multiparameter flow cytometry |
| Instrument Compatibility | Validated on analytical flow cytometers; compatible across multiple instrument platforms |
| Handling & Safety | No special handling or safety precautions required |
| Manufacturer | Slingshot Biosciences |
SpectraComp® XT Controls are precision engineered single stain cell mimics designed to optimize spectral unmixing and compensation in multiparameter flow cytometry workflows. These controls mimic the staining performance of real cells and provide clear positive and negative fluorescence peaks after staining with fluorescently conjugated antibodies.As with all SpectraComp Controls, SpectraComp XT delivers high fluorescence response and cell-like autofluorescence to provide superior signal to noise resolution and reduce errors associated with spectral overlap. The consistent fluorescence intensity and physiologically relevant scatter characteristics of SpectraComp Controls outperform traditional polystyrene based beads and support reliable unmixing across instruments and platforms. SpectraComp XT expands species compatibility by capturing antibodies from mouse, rat, hamster, rabbit and human sources, supporting broad panel design and diverse fluorophore selections. Compatible with violet, UV and tandem dyes, these controls are supplied as a combined positive and negative population for streamlined workflow integration.These controls require no special handling or safety precautions and have been validated on analytical flow cytometers for dependable performance in daily QC, assay development and high parameter panel design. Benefits: Unparalleled high fluorescence response (greater than 10e5 MFI on most bright fluorochrome) Conveniently works with multiple species of antibodies: mouse, rat, hamster, rabbit, and human Exceptional performance for unmixing/compensation across instrument platforms Compatible with violet, UV fluorochrome, and tandem dyes Consistent fluorescence signals for reliable performance Designed to support easier analysis workflows with their cell-like attributes (size, scatter, and autofluorescence) Combined positive and negative populations for ease-of-use Resources: Technical Data Sheet Safety Data Sheet Certificate of Analysis Poster: Spectral Cytometry Reference Controls: The Search for the Perfect Particle Publication: Jensen, HA, Kim, J. iCoreDrop: A robust immune monitoring spectral cytometry assay with six open channels for biomarker flexibility. Cytometry. 2023; 103(5): 405–418. https://doi.org/10.1002/cyto.a.24708
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