Immunophenotyping Controls for Flow Cytometry

TBNK Mimic™ is a lyophilized cell mimic designed for reliable performance checks and normalization controls in immunophenotyping and flow cytometry workflows. These controls feature T cell, B cell and NK cell markers with scatter characteristics that closely resemble lymphocyte, monocyte and granulocyte populations. Each control includes well‑defined populations that reflect physiologically relevant antigen densities: CD4+ T cells (CD45+, CD3+, CD4+), CD8+ T cells (CD45+, CD3+, CD8+), B cells (CD45+, CD3−, CD19+), NK cells (CD45+, CD3−, CD16/56+), classical monocytes (CD45+, CD14+) and granulocytes (CD45+). Their engineered FSC and SSC profiles closely resemble the scatter characteristics of human peripheral blood populations, enabling confident population discrimination and gating across diverse cytometry platforms. Offering superior lot‑to‑lot reproducibility and exceptional long‑term stability, TBNK Mimics outperform fresh primary cells, frozen preparations and conventional lyophilized products. With no biohazard material and no special handling or disposal requirements, they provide a dependable alternative to biologically derived controls while maintaining consistent antigen presentation and physiologically relevant scatter characteristics. This combination of stability, reproducibility and well‑defined population structure supports accurate gating, antibody performance assessment and instrument verification across daily QC, assay validation and longitudinal performance monitoring. The lyophilized format ensures consistent reconstitution and extended shelf life, making TBNK Mimics suitable for multi‑site standardization, training and long‑term assay harmonization.

Designed for positive and negative detection of naïve, central memory, effector memory, and terminal effector/exhausted T cell subsets, T Cell Memory Mimic controls are ideal for T cell enumeration, viability assessment and long‑term assay performance monitoring.

CD34+ Stem Cell Mimic™ is a lyophilized cell mimic designed for reliable performance checks and normalization controls in immunophenotyping and flow cytometry workflows. This product features CD34, CD45 and DNA‑based viability biomarkers with scatter characteristics that closely resemble human lymphocyte populations. It provides a high density CD34+ population with frequencies comparable to biological samples such as cord blood, bone marrow and mobilized peripheral blood cells (~90% CD34+, as a percentage of CD45+ cells), enabling confident population discrimination and gating. The control is intended to provide positive signal detection for CD45 and both positive and negative signal detection for CD34 and viability. It supports robust gating strategies for CD34 enumeration and viability assessment and serves as a dependable tool for antibody qualification and staining verification. The kit comprises three lymphocyte‑mimic populations designed for clear transferable gates: CD45+ cells, CD45+/CD34+ cells and CD45+ cells with a viability readout. This well‑defined population structure supports accurate gating, viability discrimination and instrument verification across daily QC, assay validation and longitudinal performance monitoring. The lyophilized format ensures consistent reconstitution and extended shelf life, making the CD34+ Stem Cell Mimics suitable for multi‑site standardization and long‑term assay harmonization across diverse cytometry platforms. Resources: Technical Data Sheet Certificate of Analysis

Slingshot Labs single biomarker CD4(+), CD4(-) are lyophilized cell mimics that feature CD4+ biomarkers with scatter properties similar to lymphocyte populations.