High-parameter flow cytometry assays demand controls that behave predictably, integrate cleanly into complex workflows, and reflect the biological context of real samples. ViaComp® Ultra™ XT - Amine is the first control of its kind: specifically developed to meet the needs of high-parameter flow cytometry by providing both antibody and viability dye unmixing and compensation capability.
Why ViaComp Ultra XT - Amine?
Traditional bead-based controls bind fluorophore-conjugated antibodies consistently, but they are not suitable for viability dye staining. Their autofluorescence and light-scatter profiles differ significantly from biological samples, often requiring adjusted instrument settings to keep control particles on scale with experimental samples. As a result, many laboratories have historically relied on partially killed or heat-shocked cells to generate viability dye controls. This approach introduces variability, increases hands-on time, and reduces standardization across operators and instruments.
ViaComp Ultra XT - Amine controls bridge that gap. Each particle is engineered to mimic key aspects of cellular behavior, including:
- Cell-like scatter and autofluorescence
- Defined amine content for viability dye evaluation
- Robust antibody binding across mouse, rat, hamster, rabbit, and human host species
This combination allows laboratories to evaluate viability dyes and antibody panels on the same control material, reducing workflow complexity and strengthening assay consistency.
Built for Workflow Integration
ViaComp Ultra XT - Amine Controls
Designed for amine-reactive viability dyes, these controls replicate the staining behavior of live and dead cells while supporting full-panel antibody compensation. Each drop of ViaComp Ultra XT - Amine contains a pre-mixed suspension of positive and negative particles, producing well-separated peaks and simplifying daily setup. The dropper-bottle format supports consistent performance across operators and instruments, while the 2 to 8°C storage and 18-month shelf life reduce logistical burden.
Where ViaComp Ultra XT - Amine Fits
ViaComp Ultra XT - Amine controls are ideal for:
- High-complexity flow cytometry panels
- Workflows that include amine-reactive viability dyes
- Assay development and QC
- Method transfer and cross-site harmonization
- Environments where biological relevance and reproducibility are essential
By providing biologically grounded spectral signatures and supporting full-panel signature control, ViaComp Ultra XT - Amine controls help laboratories build cytometry workflows that are more consistent, more reproducible, and better aligned with modern analytical expectations.
References and links
- ViaComp® Ultra™ XT - Amine product page
- ViaComp Ultra XT - Amine vs. UltraComp Spectral eBeads: choosing the right synthetic control
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